Refinement Database

Database on Refinement of Housing, Husbandry, Care, and Use of Animals in Research

This database, created in 2000, is updated every four months with newly published scientific articles, books, and other publications related to improving or safeguarding the welfare of animals used in research.

Tips for using the database:

  • This landing page displays all of the publications in the database.
  • Use the drop-down menus to filter these publications by Animal Type, Setting, and/or Topic.
  • Clicking on a parent category (e.g., Rodent) will include publications relating to all the items in that category (e.g., Chinchilla, Gerbil, Guinea Pig, etc.).
  • You may also add a keyword to further narrow your search.
  • Please note that at this time, only publications dated 2010 or later (with some exceptions) can be filtered by Animal Type and Topic, and only publications dated 2020 or later (with some exceptions) can be filtered by Setting. Most publications older than 2010 can only be searched by keyword. 
Nordquist, R., van der Staay, F., van Eerdenburg, F. et al. 2017. Mutilating procedures, management practices, and housing conditions that may affect the welfare of farm animals: Implications for welfare research. Animals 7(2), 12.

A number of mutilating procedures, such as dehorning in cattle and goats and beak trimming in laying hens, are common in farm animal husbandry systems in an attempt to prevent or solve problems, such as...

Bulens, A., Van Beirendonck, S., Van Thielen, J. et al. 2017. Hiding walls for fattening pigs: Do they affect behavior and performance? Applied Animal Behaviour Science 195, 32-37.

The prevalence of harmful and unwanted behavior in fattening pigs is influenced by different factors including housing. Housing may include the opportunity to hide from aggressive pen mates and this ability is related to pen...

McGowan, R. T. S., Ellis, J. J., Bensky, M. K. et al. 2017. The ins and outs of the litter box: A detailed ethogram of cat elimination behavior in two contrasting environments. Applied Animal Behaviour Science 194, 67-78.

Few studies have sought to describe cat elimination behavior in detail and much of the information presently available focuses on factors that potentially cause cats to reject a litter box. Thus, the ethograms published in...

van Nieuwamerongen, S. E., Mendl, M., Held, S. et al. 2017. Post-weaning social and cognitive performance of piglets raised pre-weaning either in a complex multi-suckling group housing system or in a conventional system with a crated sow. Animal Cognition 20(5), 907–921.

We studied the social and cognitive performance of piglets raised pre-weaning either in a conventional system with a sow in a farrowing crate (FC) or in a multi-suckling (MS) system in which 5 sows and...

Carreras, R., Mainau, E., Arroyo, L. et al. 2016. Housing conditions do not alter cognitive bias but affect serum cortisol, qualitative behaviour assessment and wounds on the carcass in pigs. Applied Animal Behaviour Science 185, 39-44.

Measures of animal emotions are essential to assess animal welfare. Recently, the cognitive bias technique has been proposed as a measure of animal affective state. This technique is based on the premise that subjects in...

Hales, J., Moustsen, V. A., Nielsen, M.B.F. et al. 2016. The effect of temporary confinement of hyperprolific sows in Sow Welfare and Piglet protection pens on sow behaviour and salivary cortisol concentrations. Applied Animal Behaviour Science 183, 19-27.

The aim of this study was to investigate effects of confinement in Sow Welfare and Piglet protection pens (SWAP pen) for four days after farrowing on sow behaviour and salivary cortisol levels. Sows were randomly...

Martin, J. E., Ison, S. H., Baxter, E. M. 2015. The influence of neonatal environment on piglet play behaviour and post-weaning social and cognitive development. Applied Animal Behaviour Science 163, 69-79.

Research has shown that the domestic pig is highly playful throughout its development and that play is an important aspect of social and cognitive development. Therefore, the neonatal environment is fundamental to successful stimulation of...

Verdon, M., Hansen, C. F., Rault, J. L. et al. 2015. Effects of group housing on sow welfare: a review. Journal of Animal Science 93(5), 1999-2017.

Factors that have been shown to impact the welfare of group-housed sows are discussed in this review. Floor space allowance markedly affects sow welfare. In addition to quantity of floor space, the quality of space...

Bayne, K. A., Beaver, B. V., Mench, J. A. et al. 2015. Laboratory animal behavior. In: Laboratory Animal Medicine, 3rd edition. Fox, J. G., Anderson, L., Otto, G., et al. (eds). Elsevier, Inc., Amsterdam, NL. pp. 1617-1651.

The study of laboratory animal behavior has increased steadily over the last decade, with expanding emphasis on a variety of commonly used species. In the United States, this trend was initially focused on species for...

Arhant, C., Wogritsch, R., Troxler, J. 2015. Assessment of behavior and physical condition of shelter cats as animal-based indicators of welfare. Journal of Veterinary Behavior 10(5), 399-406.

Surveillance of cat shelters by official veterinarians in Austria is based on legal requirements and does not directly assess cat welfare. The aim of this study was to develop animal-based parameters that are feasible to...

Andersen, I. L., Vasdal, G., Pedersen, L. J. 2014. Nest building and posture changes and activity budgets of gilts housed in pens and crates. Applied Animal Behaviour Science 159, 29-33.

The aim of the present work was to study nest building, posture changes and the overall activity budget of gilts in pens vs. crates. Twenty-three HB gilts (high piglet survival day 5) and 21 LB...

Stella, J., Croney, C., Buffington, T. 2014. Environmental factors that affect the behavior and welfare of domestic cats (Felis silvestris catus) housed in cages. Applied Animal Behaviour Science 160, 94-105.

Understanding environmental factors that affect the behavior of cats in cages is important if caretakers are to improve the welfare of confined cats. The aim of this study was to examine the effects of the...

Mack, L. A., Lay, D. C., Eicher, S. D. et al. 2014. Group space allowance has little effect on sow health, productivity, or welfare in a free-access stall system. Journal of Animal Science 92(6), 2554-2567.

Free-access stalls allow sows to choose the protection of a stall or use of a shared group space. This study investigated the effect of group space width, 0.91 (SS), 2.13 (IS), and 3.05 (LS) m...

Reinhardt, V. (ed.) 2013. Compassion Makes a Difference - Discussions by the Laboratory Animal Refinement & Enrichment Forum [LAREF], Volume III. Animal Welfare Institute, Washington, DC.

This is the third volume of discussions that took place on the Laboratory Animal Refinement & Enrichment Forum (LAREF). This forum is dedicated to the exchange of personal experiences of refining the conditions under which...

Baumann, S., Pflanz, W., Gallmann, E. et al. 2013. The effect of rubber mats on preference and lying behaviour of group housed sows. Landtechnik 68(6), 385-388.

This study analyses the preferences, as well as the lying behaviour, of sows with various types of mat in a group housing system lying area. The sows in the trial could choose between six bays...

Rioja-Lang, F. C., Hayne, S. M., Gonyou, H. W. 2013. The effect of pen design on free space utilization of sows group housed in gestation pens equipped with free access stalls. Applied Animal Behaviour Science 148(1-2), 93-98.

This study investigated the implementation of walk-in/lock-in stalls (free access stalls) for group housed sows. The objectives were to compare two different pen configurations (‘I-pen’ vs. ‘T-pen’) by determining the proportion and type (size/parity) of...

Spoolder, H. A. M., Aarnink, A. A. J., Vermeer, H. M. 2012. Effect of increasing temperature on space requirements of group housed finishing pigs. Applied Animal Behaviour Science 138(3-4), 229-239.

For groups of pigs to cope adequately with their housing conditions they need sufficient static space (occupied by the body of the pig), activity space (for movement between different functional areas and behaviours relating to...

Toenepoehl, B., Appel, A. K., Welp, S. et al. 2012. Effect of marginal environmental and social enrichment during rearing on pigs' reactions to novelty, conspecifics and handling. Applied Animal Behaviour Science 140(3-4), 137-145.

The rearing environment of farmed animals can affect their behaviour when handled, and therefore needs to be taken into account when selecting for traits such as docility. Therefore, 126 German Landrace and Pietrain × German...

Oostindjer, M., van den Brand, H., Kemp, B. et al. 2011. Effects of environmental enrichment and loose housing of lactating sows on piglet behaviour before and after weaning. Applied Animal Behaviour Science 134(1-2), 31-41 .

Weaning of piglets in commercial husbandry systems is earlier and more abrupt than would happen under natural conditions, resulting in low feed intakes and health and welfare problems in the immediate postweaning period. Piglets in...

Averós, X., Brossard, L., Dourmad, J. et al. 2010. A meta-analysis of the combined effect of housing and environmental enrichment characteristics on the behaviour and performance of pigs. Applied Animal Behaviour Science 127(3-4), 73-85.

To quantify the combined effect of housing conditions and environmental enrichment on the behaviour and performance of pigs, a meta-analysis was performed using information from 45 experiments in 42 published manuscripts. Multiple regression models were...

Kry, K., Casey, R. 2007. The effect of hiding enrichment on stress levels and behaviour of domestic cats (Felis sylvestris catus) in a shelter setting and the implications for adoption potential. Animal Welfare 16(3), 375-383.

Results of this study suggest that the welfare of kennelled cats is greatly improved if they are provided with the opportunity to perform effective hiding behavior, and that the ability to perform such a behaviour...

Rochlitz, I. (Ed.) 2007. The Welfare of Cats. Springer: Dordrecht, The Netherlands, 283 pp.

This book covers the major issues affecting the welfare of domestic cats. It covers recent developments in our understanding of cat behaviour, considers the ways in which cat welfare is assessed, and addresses the successes...

Hötzel, M. J., Pinheiro Machadon F., L. C., Wolf, F. M. et al. 2004. Behaviour of sows and piglets reared in intensive outdoor or indoor systems. Applied Animal Behaviour Science 86(1-2), 27-39.

In this study, we compared the behaviour of sows and piglets, as well as the mother–offspring interactions of sows and piglets during lactation, and the post-weaning behaviour of piglets reared in the outdoors or in...

Poe, E., Hope, K. 2000. Group housing products for cats. Lab Animal 29(4), 40-43.

Commercial caging systems for cats are described and recommendations made for cat-appropriate housing. By housing cats in groups and with appropriate enrichments, according to their physical and psychological needs, researchers can decrease the animal's stress...

Buré, R. G. 1981. Animal well-being and housing systems for piglets. In: The Welfare of Pigs. Sybesma, W. (ed), 198-207. Martinus Nijhoff, The Hague, Netherlands.

Weaned piglets housed with straw performed less tail-biting, rooting, massaging, and nibbling of pen-mates than pigs reared without straw.