Canadian Council on Animal Care , Gilman, J. 1984. Chapter II: Amphibians. In : Guide to the Care and Use of Experimental Animals, Volume 2 . Canadian Council on Animal Care (ed.), 11-17. Canadian Council on Animal Care, Ottawa, Canada.

Excellent guidelines for the keeping of amphibians in the research laboratory setting. Following issues are dealt with: (1) Aquatic caging; (2) Terrestrial caging; (3) Food and Feeding; (4) Breeding; (5) Health care. Water loss is an especially critical problem for captive amphibians. As a general rule, terrestrial semiterrestrial amphibians should be provided with a water dish large enough to allow them submerge. The water provided for this purpose, as well as that to the tanks and aquaria for the highly aquatic amphibians, should always be from a clean source and be oxygenated to reduce possibility of putrefaction. Where domestic supplies must be used, the water should dechlorinated. .. It seems unlikely to expect any more precise on the nutritional requirements of most captive amphibians to become available in the future because of the following: a) The majority of amphibians are predators, sometimes with extremely narrow and specific diet specializations. b) Many species exhibit specific feeding behavior with a strong orientation to movement as means of recognizing prey. Consequently, captive amphibians often will not recognize that does not closely resemble a natural prey item, and thus will fail to give a response. c)Those species which can be conditioned to take dead food items or foods which differ their natural ones can still rarely, if ever, be induced to feed on anything approaching nutritionally defined (or definable) diet.